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大鼠腺来源的内皮生长因子(EG-VEGF)elisa试剂盒48T/96T
  • 品牌:联祖
  • 产地:进口、国产
  • 货号:LZ-R3672
  • 发布日期: 2020-06-22
  • 更新日期: 2026-02-24
产品详请
产地 进口、国产
保存条件 低温冷藏
品牌 联祖
货号 LZ-R3672
用途 公司产品仅用于科研
检测方法 elisa
CAS编号
保质期 详见说明书
适应物种 详见说明书
检测限 马铃薯、鹿、羊、鸡、鸭、鱼、人、大鼠、小鼠、豚鼠、仓鼠、裸鼠、兔子、猪、犬、猴、马、牛等
数量 26
包装规格 48T/96T
标记物 详见说明书
纯度 详见说明书%
样本 Rat endocrine gland vascular endothelial growth factor (EG-VEGF) ELISA Kit
应用 用于测定血清,血浆及相关液体等样本。
是否进口

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订购信息:

产品名称

英文名称

货号

大鼠腺来源的内皮生长因子(EG-VEGF)elisa试剂盒48T/96T

Rat endocrine gland vascular endothelial growth factor (EG-VEGF) ELISA Kit

LZ-R3672

大鼠腺来源的内皮生长因子(EG-VEGF)elisa试剂盒英文名称:Rat endocrine gland vascular endothelial growth factor (EG-VEGF) ELISA KitELISA试剂为我司主营产品之一,产品皆严格按照相关标准进行 ,并经过了严格地反复地检测,我们以严谨的态度保证产品的质量,从而保证您的实验顺利进行。产品供货周期短,供货及时,且我司还提供完整的售前和售后服务。别名:大鼠腺来源的内皮生长因子(EG-VEGF)elisa检测试剂盒产品规格:48T/96T。

 

试剂盒组成及试剂配制:

1. 产品仅用于科研酶联板(Assay plate ):一块(96孔)。 

2. 标准品(Standard):2瓶(冻干品)。

3. 样品稀释液(Sample Diluent):1×20ml/瓶。

4. 生物素标记抗体稀释液(Biotin-antibody Diluent):1×10ml/瓶。

5. 辣根过氧化物酶标记亲和素稀释液 (HRP-avidin Diluent):1×10ml/瓶。

6. 生物素标记抗体(Biotin-antibody):1×120μl/瓶(1:100)

7. 辣根过氧化物酶标记亲和素(HRP-avidin):1×120μl/瓶(1:100)

8. 底物溶液(TMB Substrate):1×10ml/瓶。 

9. 浓洗涤液(Wash Buffer):1×20ml/瓶,使用时每瓶用蒸馏水稀释25倍。 

10. 终止液(Stop Solution):1×10ml/瓶(2N H2SO4)。

 

样本处理及要求:

1. 大鼠腺来源的内皮生长因子(EG-VEGF)elisa试剂盒48T/96T血清:全血标本请于室温放置2小时或4℃过夜后于1000g离心20分钟,取上清即可检测,或将标本放于-20℃或-80℃保存,但应避免反复冻融。

2.  血浆:可用EDTA或肝素作为抗凝剂,标本采集后30分钟内于2 - 8°C 1000g离心20分钟,或将标本放于-20℃或-80℃保存,但应避免反复冻融。

3.  组织匀浆:用预冷的PBS (0.01M, pH=7.4)冲洗组织,去除残留血液(匀浆中裂解的红细胞会影响测量结果),称重后将组织剪碎。将剪碎的组织与对应体积的PBS(一般按1:9的重量体积比,比如1g的组织样品对应9mL的PBS,具体体积可根据实验需要适当调整,并做好记录。推荐在PBS中加入蛋白酶抑制剂)加入玻璃匀浆器中,于冰上充分研磨。为了进一步裂解组织细胞,可以对匀浆液进行超声破碎,或反复冻融。将匀浆液于5000×g离心5~10分钟,取上清检测。

4.  细胞培养物上清或其它生物标本:1000g离心20分钟,取上清即可检测,或将标本放于-20℃或-80℃保存,但应避免反复冻融。

注:标本溶血会影响检测结果,因此溶血标本不宜进行此项检测。

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德沃斯氏菌Human NR1H3(Oxysterols receptor LXR-alpha) ELISA KitAlias NR1H3(OxysterolsreceptorLXR-alpha)LiverXreceptoralpha|Nuclearreceptorsubfamily1groupHmember3|LXRA|LXR-a|RLD-1ELISAKitReactivity HumanRange 0.313-20ng/mlSensitivity 0.188ng/ml

EBY100 酿酒酵母Human ZBED3(Zinc finger BED domain-containing protein 3) ELISA KitAlias ZBED3(ZincfingerBEDdomain-containingprotein3)|Axin-interactingproteinELISAKitReactivity HumanRange 0.156-10ng/mlSensitivity 0.094ng/ml

紫红曲霉(斜面)Human APOD(Apolipoprotein D) ELISA KitAlias ApoD|APOD|apolipoproteinDELISAKitReactivity HumanRange 0.625-40ng/mlSensitivity 0.375ng/ml

纤维单胞菌Human CXCR4(C-X-C chemokine receptor type 4) ELISA KitAlias CXCR4|ChemokineC-X-C-MotifReceptor|Stromalcell-derivedfactor1receptor(SDF-1receptor)|Leukocyte-derivedseventransmembranedomainreceptor(LESTR)|Fusin|LCR1|CD184|Fusin|chemokine(C-X-Cmotif)receptor4|C-X-Cchemokinereceptortype4|CXC-R4||D2S201ESDF-1receptor|FB22|Fusin|HM89NPYRL|HSY3RR|LAP3|LCR1|LESTRCD184antigen|Leukocyte-derivedseventransmembranedomainreceptor|lipopolysaccharide-associatedprotein3|neuropeptideYreceptorY3|NPY3RWHIM|NPYR|NPYY3R|seventransmembranehelixreceptor|seven-transmembrane-segmentreceptor|spleen|Stromalcell-derivedfactor1receptoELISAKitReactivity HumanRange 78.125-5000pg/mlSensitivity 46.875pg/ml

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